The requirement for liganddependent processing for Notch activity was verified using mutants disabled for interactions with ligand. The recombinant Notch1LNG, which lacks the epidermal growth factor (EGF)like repeats in its extracellular domain,20 did not block death (Figure 2b), whereas a construct corrected for this defect (Notch1LNG CCSS) imparted protection from death (Figure 2b). As expected, only Notch1LNG CCSS activated the Hes1 promoter (a readout of transcriptional activity) in reporter assays (Figure 2d).Money was originally created as a means of exchange that would simplify the barter process. Instead of my having to carry chickens around with me that I could trade for baked goods or clothing or shelter, the invention of money means I get to sell my chickens for money and use the money to get the cupcake, sweater, or house. Those goods or services I would love to offer that will make a positive difference in the lives of others I can now exchange them directly for cash.